Negative: < 1:40 titer
Positive: ≥ 1:40 titer
|Schedule Turnaround Time||Assay performed daily Mon.-Fri. Report availability is within 48 hours from the time of specimen receipt.
|Specimen Requirements||Specimen need not be refrigerated or frozen. Collect 5-10 ml of blood in a red
top or serum separator tube. If possible, separate serum from clot and place into orange tube provided with
IMMCO collection kits. Do not puncture top of orange tube. If separation facilities are not available, the
blood can be sent in the tube used for collection.
|Sample Stability||Sample is stable at ambient temperature during shipment. If sample is stored prior to shipment, it is stable refrigerated (2-8°C) up to five days and frozen (-20°C or lower) up to one year.
|Clinical Relevance||For detection of antibodies to nuclear antigens. The homogeneous pattern is primarily associated with systemic lupus erythematosus (SLE). Antibodies to centromere antigens are highly specific for CREST syndrome, a limited form of systemic sclerosis. Centromere antibodies can also occasionally befound in low titers in some other autoimmune variants. Anti-nuclear antibodies with a speckled pattern are commonly associated with systemic lupus erythematosus (SLE), although they do occur in some cases of Sjögren’s syndrome and mixed connective tissue disorders. ANA antibodies with a nucleolar pattern are
commonly associated with systemic sclerosis (SS), although they do occur in some cases of systemic lupus
erythematosus (SLE) and overlap syndromes. Overlap syndromes include polymyositis/dermatomyosistis.
- Autoimmune Gastritis
- Autoimmune Hepatitis
- Neuropsychiatric Lupus
- Rheumatoid Arthritis
- Sensorineural Hearing Loss
- Sjögren's Syndrome
- Systemic Lupus Erythematosus (SLE)